AmScope ME320TWB-PZ-2L-5M Digital Polarizing Trinocular Metallurgical Microscope, WF10x and WF20x Eyepieces, 40X-2000X Magnification, Episcopic and Diascopic Halogen Illumination with Rheostat, Double-Layer Mechanical Stage, Sliding Head, High-Resolution Optics, 90-240V, Includes 5MP Camera with Reduction Lens and Software

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The AmScope ME320TWB-PZ-2L-5M digital polarizing metallurgical trinocular microscope has interchangeable pairs of 10x and 20x widefield eyepieces, a forward-facing triple nosepiece with three metallurgical and four Brightfield objectives, episcopic and diascopic halogen illumination, and a double-layer mechanical stage with a stage stop to protect slides and objectives from damage. The 5MP camera has a CMOS color sensor, a reduction lens, image capture and editing software, and USB 2.0 output to capture or display still or video images on a computer or projector. The trinocular viewing head has a 23mm vertical camera mount, a Siedentopf binocular head to adjust interpupillary distance, a fixed 30-degree vertical inclination to reduce eye and neck strain, and a 360-degree rotation capability to enable sharing. The Siedentopf head has a range of 55 to 75mm to accommodate individual eye differences, and bilateral dioptric adjustment to accommodate individual eye-strength differences. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to re-focus the image. The microscope has a forward-facing nosepiece and includes three metallurgical objectives and four Brightfield achromatic objectives. The 5x, 10x, and 50xS plan achromatic metallurgical objectives combine with the eyepieces to provide color correction of magnified images. Plan objectives provide improved focus over the entire range of the viewing field. The 4x, 10x, 40xS (spring), and 100xS (oil) DIN achromatic objectives provide color correction of magnified images. The 40xS and 50xS objectives are spring loaded to prevent slide damage when focusing. The 100xS spring-loaded oil-immersion objective uses oil between the specimen and the objective lens to provide increased resolution over a standard objective. A digital polarizing metallurgical microscope uses transmitted and reflected light and a polarizing filter to view metallurgical specimens, including electronics, or larger specimens not viewable on a standard microscope stage, and is used where image capture, detailed records, or documentation is required.

The 5MP digital camera has a CMOS color sensor for displaying still microscopy images and streaming live videos to a computer or projector. The camera has 40x magnification and a 0.5x reduction lens that ensures that the display has a similar field of view as the microscope eyepiece. The camera can be mounted in a C-Mount or any 23mm eye tube. The camera includes image capture and editing software that provides still image and live video capture and editing capability, including measurement functions. The software supports JPG, TIF, GIF, PSD, WMF, and BMP file formats and is compatible with Windows XP, Vista, 7, and 8; Mac OS X; and Linux. Camera drivers are compatible with Windows XP, Vista, 7, and 8; Mac OS X; and Linux. The software includes Windows APIs for native C/C++, C#, DirectShow, Twain, and LabVIEW that enable custom application development. The camera has a USB 2.0 data port (cable included).

The microscope has reflected episcopic (upper) illumination that reflects light off the specimen for enhanced visibility of opaque specimens, and transmitted diascopic (lower) illumination that transmits light up through the specimen for enhanced visibility of translucent and transparent objects. The episcopic illumination system has independent filter holders for yellow, blue, green, and frosted filters (included). The 30W halogen light source provides bright light in a concentrated path and has a rheostat to control light intensity. Halogen illumination provides bright light in a concentrated path. The double-layer mechanical stage, with 1mm stage divisions and 0.1mm vernier graduations, locks the slide into place and provides precise slide manipulation along the X- and Y-axis to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. A stage stop prevents the stage from coming into contact with the slide and objectives. The stage is 7 x 5-3/4 inches (180 x 145mm) and has a traveling range of 1-3/16 x 2-3/4 inches (30 x 70mm). Graduated coaxial coarse and fine focus has a focusing range of 1-9/16″ (40mm). Focus knob tension control prevents the stage from drifting out of focus. The microscope has a mechanical tube length of 6-5/16″ (160mm).

Microscope Specifications
HeadTrinocular
Magnification40x-2000x
Trinocular port23mm
Eyepieces (23mm)WF10x18mm, WH20x10mm
Objectives, metallurgical (20mm)5x, 10x, 50xS DIN plan achromatic
Objectives, Brightfield (20mm)4x, 10x, 40xS, 100xS (oil) DIN achromatic
StageDouble-layer mechanical
FocusBilateral nested coarse and fine
Light source30W halogen with rheostat
Illumination typeEpiscopic polarizing and diascopic
FilterBlue
Power90 to 240V, CE certified

Camera Specifications
Resolution5MP (2592 x 1944 effective pixels)
Image typeStill image and video display and capture
Camera typeBrightfield
Camera sensor1/2.5″ Aptina MT9P001 CMOS (color)
Magnification40x
Reduction lens0.5x
Mounting size23mm or C-Mount
Frame rate60fps at 640×480; 18fps at 1280×960; 5fps at 2592×1944
Computer connectionUSB 2.0 (backward compatible on PCs only)
File formatsJPG, TIF, GIF, PSD, WMF, BMP
Software packageImage capture and editing for Windows XP, Vista, 7, and 8; Mac OS X; and Linux
Camera driver compatibilityWindows XP, Vista, 7, and 8; Mac OS X; and Linux

Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.

United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.

What’s in the Box?

  • AmScope ME320TWB-PZ-2L-5M microscope with mechanical stage
  • WF10x eyepieces, 23mm, one pair
  • WF20x eyepieces, 23mm, one pair
  • 5x DIN plan achromatic metallurgical objective, 20mm
  • 10x DIN plan achromatic metallurgical objective, 20mm
  • 50xS DIN plan achromatic metallurgical objective, 20mm
  • 4x DIN achromatic objective, 20mm
  • 10x DIN achromatic objective, 20mm
  • 40xS DIN achromatic objective, 20mm
  • 100xS (oil) DIN achromatic objective, 20mm
  • Polarizing illumination system
  • (4) Color filters: yellow, blue, green, and frosted
  • 5MP digital camera (MU500)
  • 0.5x reduction lens
  • USB 2.0 cable
  • Software CD
  • Immersion oil
  • Instructions

Digital polarizing metallurgical microscope uses transmitted and reflected light and a polarizing filter to view metallurgical specimens, including electronics, or larger specimens not viewable on a standard microscope stage, and a 5MP camera with reduction lens and USB 2.0 output for capturing or displaying images on a computer or projector
Trinocular head with interchangeable pairs of 10x and 20x widefield eyepieces, Siedentopf binocular head with 55 to 75mm interpupillary adjustment, fixed 30-degree vertical inclination to reduce eye and neck strain, and 360-degree rotation capability to enable sharing
Forward-facing triple nosepiece with 5x, 10x, and 50xS plan achromatic metallurgical objectives for improved focus over the entire field of view; and 4x, 10x, 40xS, and 100xS (oil) DIN achromatic objectives that provide color correction of magnified images
Episcopic polarizing and diascopic illumination system has 30W halogen illumination with rheostat to control light intensity
Double-layer mechanical stage has 1mm stage divisions with 0.1mm vernier graduations and stage stop; graduated coarse and fine focus with tension control to prevent stage drift

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